Using Sound Waves for cGMP Manufacturing of a Fusion Protein with Mammalian Cells
نویسنده
چکیده
biopharmaceuticals continues to increase from an estimated $12 billion three years ago to $30 billion today. Of the 122 biopharmaceutical products approved in the United States and Europe, 50 are produced using mammalian cells, 39 by prokaryotic cells, 21 using a yeast system, and a further 12 products by undisclosed systems (1). As an increasing number of potential new drugs emerge from the preclinical stage into clinical trials (now estimated at approximately 500 undergoing clinical evaluation), demand for the cGMP manufacture of these products continues to increase. The main methods of making such products are batch, fed-batch, and continuous perfusion. The mode of
منابع مشابه
IMPROVEMENT OF hGM-CSF EXPRESSION USING A FUSION SYSTEM BY DIRECTING PROTEIN TO THE PERIPLASMIC SPACE
Bacterial expression systems provide economic and logistic advantages in manufacturing proteins for human therapeutic purposes. However, most such proteins accumulate in insoluble biologicaIIy inactive form when overexpressed in bacterial cells. This is the case while attempting to produce recombinant human granulocyte-macrophage colony stimulating factor (hGM-CSF) in E. coli. In this repor...
متن کاملIn vivo Characterization of Fusion Protein Comprising of A1 Subunit of Shiga Toxin and Human GM-CSF: Assessment of Its Immunogenicity and Toxicity
Background: Most cancer cells become resistant to anti-cancer agents. In the last few years, a new approach for targeted therapy of human cancer has been developed using immunotoxins which comprise both the cell targeting and the cell killing moieties. Methods: In the present study, the recombinant Shiga toxin A1 subunit fused to human granulocyte-macrophage colony stimulating factor (A1-GM-CSF...
متن کاملEffects of Sound Stimulation on Physiological and Biochemical Responses of ‘Salvia splendens’
Plants are influenced inevitably by environmental stresses due to their immobility. Sound waves are one of these environmental factors affecting plants. This study was designed to understand the relationship between sound waves and plant responses that is still low knowledge. For this purpose, Salvia splendens, one of the common plants in urban green spaces, were influenced by sound wa...
متن کاملConstruction of Hybrid Gene of Hepatitis B Surface Antigen Carrying Heat-Stable Enterotoxin of Escherichia coli and Its Expression in Mammalian Cell Line
Hepatitis B surface antigen is the first genetically engineered vaccine licensed for human use. Various strategies have been proposed to obtain a vaccine that would bypass the need for injection. In this study, a non-toxic portion of heat-stable enterotoxin of Escherichia coli that is capable of adhering to epithelial cells was inserted at amino acid position 112 of hepatitis surface antigen. T...
متن کاملA novel chimeric recombinant protein PDHB-P80 of Mycoplasma agalactiae as a potential diagnostic tool
The aim of this study was to construct, expression of a novel recombinant chimeric protein consisting of Pyruvate dehydrogenase beta subunit (PDHB) and high antigenic region of integral membrane lipoprotein P80 of Mycoplasma agalactiae as a potential diagnostic tool. The full-length sequence of pdhb and a portion of antigenic regions of P80 were selected and analyzed by CLC ma...
متن کامل